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. 2023 May 4;10:1085578. doi: 10.3389/fmed.2023.1085578

Figure 1.

Figure 1

LGG postbiotic prevents mTOR pathway activation induced by PTG and P31-43 in Caco-2 cells. (A, D) Caco-2 cells untreated (NT), after being treated with P31-43 and PTG for 1 h, and pretreated with LGG postbiotic were analyzed by WB and were stained with antibodies against pmTOR, pp70S6K, and p4EBP. Tubulin and GAPDH were used as loading controls. The immunoblotting analysis is representative of three independent experiments. (B, C, E) Densitometric analysis of bands from WB as shown in (A, D). Columns represent the mean and the bars represent the standard deviation of the relative intensity of pmTOR, pp70S6K, and p4EBP with respect to total tubulin protein. Student's t-test: *p < 0.05; **p < 0.01.