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. 2023 May 4;10:1085578. doi: 10.3389/fmed.2023.1085578

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Copyright © 2023 Furone, Bellomo, Carpinelli, Nicoletti, Hewa-Munasinghege, Mordaa, Mandile, Barone and Nanayakkara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LGG postbiotic decreased NF-κβ activation after treatment with P31-43 and PTG. (A) Caco-2 cells untreated (NT), after being treated with P31-43 and PTG for 1 h, and pretreated with LGG postbiotic were analyzed by WB and were stained with antibodies against pNF-κβ. GAPDH was adopted as a loading control. The immunoblotting analysis is representative of three independent experiments. (B) Densitometric analysis of bands from WB as shown in (A). Columns represent the mean and the bars represent the standard deviation of the relative intensity of pNF-κβ with respect to the total GAPDH protein. Student's t-test: ^**p < 0.01; ^***p < 0.001.