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. 2023 May 4;10:1085578. doi: 10.3389/fmed.2023.1085578

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Copyright © 2023 Furone, Bellomo, Carpinelli, Nicoletti, Hewa-Munasinghege, Mordaa, Mandile, Barone and Nanayakkara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LGG postbiotic decreased NF-κβ phosphorylation in GCD-CD intestinal organoids (A) Protein lysates from CTR organoids untreated (NT), treated with LGG postbiotic, and analyzed by WB, were stained with antibodies against pNF-κβ. GAPDH was adopted as a loading control. The immunoblotting analysis is representative of three independent experiments. (B) Densitometric analysis of bands from WB as shown in (A). Columns represent the mean and the bars represent the standard deviation of the relative intensity of pNF-κβ with respect to the total GAPDH protein. (C) Western blot analysis of protein lysates from GCD-CD organoids untreated (NT) and treated with LGG postbiotic were probed with antibodies against PNF-κβ. GAPDH was adopted as a loading control. The immunoblotting analysis is representative of four independent experiments. (D) Densitometric analysis of bands from WB as shown in (C). The columns represent the mean and the bars represent the standard deviation of the relative intensity of PNF-κβ with respect to the total GAPDH protein. Student's t-test: ^*p < 0.05.