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. 2023 May 17;6(8):e202201685. doi: 10.26508/lsa.202201685

Figure 1. Regulation of p27 expressions by differential Cx37 constructs.

Figure 1.

(A) Localization of GFP-tagged Cx37 constructs and corresponding p27 immunofluorescence in HUVEC. Arrowhead indicating the localization of Cx37 constructs on the membrane and at cell–cell junctions. (B, C, D) Quantification of p27 immunofluorescence and the expression profile of p27 mRNA and protein in Cx37-GFP constructs in HUVEC, values normalized to control (GPF-empty vector-expressing HUVEC). (E, F) Edu fluorescence and % of EdU-positive cell quantitation (F) indicating the effect of different Cx37 constructs’ expression on cell proliferation. (G) Expression of HA-tagged Cx37 constructs. Control indicating the HUVEC-expressing HA-empty vector. (H) Schematic image of HUVEC-FastFUCCI reporter distinguishes cell cycle stages. (I) HA-Cx37FL expression increases cdt1-RFP-positive nucleus population in HUVEC-FastFUCCI cells compared with the HA-control cells. (J) HA-Cx37 constructs expression alters HUVEC-FastFUCCI cell cycle states. (K) Effect of HA-Cx37FL overexpression on p27-KD HUVEC-FastFUCCI cell cycle. (B, C, D, F, J, K) One-way ANOVA (B, C, D, F) with Dunnett’s multiple comparisons test, two-way ANOVA (J, K) with Sidak’s multiple comparisons test. Scale bar: 20 μm (A) and 100 μm (E, I).