(A, B, C, D) Effect of FSS on relative localization of Foxo3a and pERK; immunofluorescence (A, C) and quantification (B, D). (E, F) Effect of Cx37 knockdown on ERK and pERK protein level; immunoblot (E) and quantitation (F). (G) Effect of Foxo3a KD and kinase inhibitors on FSS-induced HUVEC-FastFUCCI cell cycle. (H) In Cx37 KD background, the effect of HA-CX37FL and ERK-binding mutant of Cx37; HA-Cx37ΔCT on arterial genes expression in FSS-induced HUVEC. (I) The effect of Foxo3a KD on arterial genes expression in FSS-induced HUVEC. Two-way ANOVA (B, D, F, G, H, I) with Sidak’s multiple comparisons test. Scale bar: 40 μm (A, C).