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. 2023 May 17;6(8):e202201685. doi: 10.26508/lsa.202201685

Figure S2. Regulation of p27 expressions by differential Cx37 constructs.

Figure S2.

(A) MTT cell viability assay of Cx37 constructs expressing HUVEC after 96 h of transduction. (B, C) scrape-loading dye transfer GJIC assay in Cx37 construct-expressing HUVEC, fluorescence images of Lucifer yellow, rhodamine dexran, and DAPI after 10 min of dye translocation after monolayer scratching (B), and quantitation of the dye translocation distance as a fraction of control; for more details see the methods (C). (D) FACS gating of HUVEC-FastFUCCI reporter. (E) si-RNA-mediated p27 knockdown in HUVEC. One-way ANOVA (A, C) with Dunnett’s multiple comparisons test. (F) Two-way ANOVA (F) with Sidak’s multiple comparisons test. Scale bar: 100 μm (B).