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. 2023 May 17;6(8):e202201685. doi: 10.26508/lsa.202201685

Figure S3. Nuclear stabilization of Foxo3a by Cx37 and p27 up-regulation.

Figure S3.

(A, B) Relative mRNA expression of Foxo3a and MEN1 in Cx37FL-GFP-expressing HUVEC, when control cells are expressing GFP-Empty vector. (C, D) Foxo3a Knockdown with two different human-specific shRNA and quantitation showing equivalent knockdown effect with them (D). (E, F) Immunoblots and quantitation (F) showing the effect of Foxo3a KD and expression of HA-Foxo3a in Foxo3a KD cells on p27 protein level in Cx37LF-GFP expression background. Foxo3aKD cells are expressing Ha-Empty vector. (G) mRNA expression profile of c-Myc in Cx37FL expressing HUVEC. (H, I) immunoblot and quantitation (I) showing the effect of Foxo3a on cMYC protein level with or without Foxo3a KD in steady state and Cx37FL-GFP expressing background. (J, K) Effect of PI3K (LY294002) and ERK kinase inhibitor (U0126) on Foxo3a spatial fluorescence intensity, immunofluorescence (J), and quantitation (K). (A, B, D, F, G, I) Unpaired t test with Welch correction (A, B, G), one-way ANOVA (D, F, I) with Dunnett’s multiple comparisons test. Scale bar: 20 μm (F).