Fig. 6.

In vitro models constructed on various types of NF membrane-integrated culture platforms. (a) Microfluidic skin model constructed on NF microfluidic chip and its (i) schematic illustration and (ii) confocal microscopic images showing 3D view and top view of the channel. HaCaT cells stained with both phalloidin (magenta) and DAPI (blue) and NIH3T3 cells in type I collagen hydrogel layer stained with only DAPI (blue). (b) Blood–brain barrier coculture model constructed on NF insert and its (i) schematic illustration and (ii) confocal microscopic images showing top view and cross-section view. iPSC-derived brain microvascular endothelial cells stained with both ZO-1 (red) and DAPI (blue) and astrocytes stained with GFAP (green). (c) 3D iPSC spheroid model constructed on NF microwell insert and its (i) schematic illustration, (ii) microscopic image, and (iii) confocal microscopic image of iPSC cells stained with calcein AM (green) and propidium iodide (red). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)