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. 2023 Apr 26;26(5):106748. doi: 10.1016/j.isci.2023.106748

Figure 5.

Figure 5

Ablation of whole pancreas Dpp4 does not improve glucose tolerance or GSIS

(A) Immunofluorescence staining of glucagon (purple) and DPP4 (green) and insulin (purple) and DPP4 (green) in HFD-fed mouse pancreatic sections of PDX-Cre and Dpp4Pan−/−. DAPI staining (blue) was used to identify nuclei.

(B) Absolute mRNA abundance of Dpp4 in islets isolated from one-year-old control (Dpp4(fl/fl)) and Dpp4Pan−/− male and female mice.

(C and D) Glycemia and AUC glucose during (C) oral and (D) i.p. glucose tolerance tests in HFD-fed control and Dpp4Panl−/− male mice.

(E) Perifusion GSIS in male mice fed HFD for 25–30 weeks, with and without Exendin 9-39.

(F and G) Glycemia and AUC glucose during (F) oral and (G) i.p. glucose tolerance tests in HFD-fed control and Dpp4Pan−/− female mice.

(H) Perifusion GSIS in female mice fed HFD for 25–30 weeks, with and without Exendin 9-39 (100 nM). Statistical analysis was done with mixed-effects analysis and Tukey’s multiple comparisons (C–H), one-way ANOVA (AUC E and H) or unpaired t test (B, AUC C, D, F and G). All data are represented as the mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Scale bars represent 50 μm.