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. 2023 May 4;78:100208. doi: 10.1016/j.clinsp.2023.100208

Fig. 4.

Fig 4

TPPU promoting tube forming and angiogenesis. (A) In vitro HUVECs (pretreated with/without TPPU) tube formation assay, scale bar = 200 μm. (B) Quantification of cumulative tube length and nodes for tube formation. (C) Immunohistochemical staining showing the CD31 expression in NS, TPPU-treated and PBS-treated groups, respectively, scale bar = 20 μm. (D) Quantification of CD31 expression. (E) Immunohistochemical staining showing the VEGF expression in NS, TPPU-treated and PBS-treated group, respectively, scale bar = 20 μm. (F) Quantification of VEGF expression. NS, normal SD rat without any treatment. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.