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Analysis of Gac synthesis in mutants by Western blotting. Whole-cell B. burgdorferi lysates resolved by sodium dodecyl sulfate–12.5% polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue (panel A). Fractionated lysates were electroblotted to polyvinylidene difluoride and were probed with an anti-Gac polyclonal antiserum (panel B) or an anti-Hbb polyclonal antiserum (panel C). Molecular mass standards are shown on the left in kilodaltons.
