FIG. 3.

Effect of Gac on growth of B. burgdorferi. Growth was assayed essentially as described previously (12). Cultures of B. burgdorferi strains B31 (●), B31-NGR (○), CKO-1 (▵), and CKO-2 (▾) were inoculated with the same number of cells into BSK-H medium and were grown for 6 days. One milliliter of each culture was removed each day, cells were pelleted and resuspended in 1 ml of Dulbecco's phosphate-buffered saline, and the optical density at 600 nm was determined. Plotted are the means of five independent experiments (four for days 4 and 6); error bars represent standard deviations.