Figure 2.

Brain insulin receptor expression is required for the suppression of WAT lipolysis (A) Blood glucose levels of IR^ΔPER mice and controls^ΔPER prior to and during an 8mU hyperinsulinemic clamp. (B) Blood glucose levels of IR^ΔWB mice prior to and during a 4mU (controls^ΔWB) and 32mU (IR^ΔWB) hyperinsulinemic clamp. (C) Schematic illustration of the hyperinsulinemic clamps and glucose infusion rates (GIR) of IR^ΔPER mice and controls^ΔPER. (D) Schematic illustration of the hyperinsulinemic clamps and glucose infusion rates (GIR) of IR^ΔWB mice and controls^ΔWB. (E) Rate of appearance (Ra) of glycerol of IR^ΔPER mice and controls^ΔPER at baseline and during the hyperinsulinemic clamp. (F) Ra glycerol of IR^ΔWB mice and controls^ΔWB at baseline and during the hyperinsulinemic clamp. (G) Percentage Ra glycerol suppression during the clamp in IR^ΔPERmice, IR^ΔWBmice and respective controls after the onset of the hyperinsulinemic clamps normalized to the insulin dose during the clamps (i.e. 8mU for IR^ΔPER and control^ΔPER, 4mU for control^ΔWB and 32mU for IR^ΔWB) (H) Plasma non-esterified fatty acids (NEFA) of IR^ΔPER mice and controls^ΔPER at baseline and during the hyperinsulinemic clamp. (I) Plasma NEFA of IR^ΔWB mice and controls^ΔWB at baseline and during the hyperinsulinemic clamp. Data are shown as mean ± SEM. n ≥ 5. ∗p < 0.05, ∗∗p < 0.01 ∗∗∗p < 0.001 by Student's unpaired t test (A, B, C, D, G and H) and paired t test (E, F, H and I).