FIG. 5.

Chromosomal locations of the MPR1 and MPR2 genes in S. cerevisiae strains. (A) Pulsed-field gel electrophoresis of genomic DNAs from S. cerevisiae strains. Electrophoresis was carried out as described in Materials and Methods, and the gel was stained with ethidium bromide. CHR, chromosome. (B) Southern hybridization. After electrophoresis, each genomic DNA was transferred onto a nylon membrane and hybridized with a 1,636-bp fragment for the MPR1 gene labeled using the ECL direct nucleic acid labeling system. Lane 1, Σ1278b; lane 2, FH506; lane 3, MB329-17C; lane 4, S288C; lane 5, XU-1. (C) Schematic map of the MPR1 gene on chromosome XIV (left) and the MPR2 gene on chromosome X (right) of strain Σ1278b. Predicted transcriptional orientation of MPR1 or MPR2 is shown by an arrow.