FIG. 6.
Growth phenotype of S. cerevisiae strains on minimal medium containing AZC. Approximately 106 cells of each strain and serial dilutions of 10−1 to 10−3 (from left to right) were spotted onto SD plates with appropriate amino acids in the absence (−AZC) and presence (+AZC) of AZC. Plates were incubated at 30°C for 3 days. (A) Function of the MPR1 and MPR2 genes in Σ1278b background strains. The MPR1 and MPR2 disruptants derived from strain FH506 are represented by FH506D1 and FH506D2, respectively. The MPR1 MPR2 double disruptant is represented by FH506D12. (B) Function of the MPR1 gene in the other S. cerevisiae strains. Plasmids pMH1 and pMH3 were constructed from vectors pYES2 and pRS406, respectively.