Figure 3.

Comparison of preservation of retinal thickness with various promoters

Measurements of retinal thickness measured from outer plexiform layer (OPL) to the retinal pigment epithelium (RPE) at 2 months (A) and 4 months (B) of age. Measurements for Nmnat1^V9M/V9M untreated retinas represent the average retinal thickness for the untreated contralateral eye for all groups. Wild-type (WT) untreated measurements represent the average retinal thickness for the untreated contralateral eye from all WT groups. Measurements represent the thickness in the inferior region of the retina (closest to the injection site). All treated eyes were treated with scAAV9 with the respective promoter driving expression of hNMNAT1. This virus was delivered at 1E9 vg/μL and co-injected with AAV9 CMV.GFP at a titer of 1E8 vg/μL. The red dashed line represents the mean thickness of the untreated Nmnat1^V9M/V9M retinas at the respective age. Statistically significant preservation of retinal thickness between untreated and treated Nmnat1^V9M/V9M retinas are noted by red asterisks (n $\ge$ 3 WT and n $\ge$ 5 Nmnat1^V9M/V9M mice for each group at each age, two-way ANOVA, multiple comparisons, p < 0.05, error bars represent the SEM, points represent the respective retinal thickness for each eye from a single mouse).