FIG. 3.
Regulation of atsR::lacZ and atsB::lacZ expression in P. aeruginosa PAO1S. Cells were grown in succinate-minimal medium with pentanesulfonate (Pn), methionine (Met), nitrocatechol sulfate (NCS), hexyl sulfate (Hx), or sulfate (SO4) as the sulfur source and were harvested in the mid-exponential phase. β-Galactosidase specific activity (spec. act.) was measured with ONPG as the substrate. ░⃞, atsR::lacZ (pME4334); □, atsB::lacZ (pME4337); ■, vector control (pQF120).