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Effect of cysB on atsB and atsR expression in P. aeruginosa. Cells were grown in succinate-minimal medium with methionine (Met), thiocyanate (SCN), or both as the sulfur sources and were harvested in the mid-exponential phase. Expression of atsB::lacZ and atsR::lacZ was measured as β-galactosidase activity in the wild-type strain PAO1S or in the cysB mutant PAO-CB. ░⃞, atsR::lacZ (pME4333); □, atsB::lacZ (pME4337); ■, vector control PAO1S(pQF120).
