(A) Human as reference. Left: Box plots with overlaid data points show the number of annotated orthologs. Non-placental mammals are highlighted with a yellow background. Right: Box plots showing evolutionary distances to human.
(B) Mouse as the reference. Muridae are shown as a separate group.
(C) TOGA with chicken as the reference, applied to 501 bird assemblies.
(D) TOGA for other species using NCBI RefSeq annotations (21) as the reference. BUSCO gene completeness of the reference annotation provides an upper bound for the completeness of TOGA’s query annotation.