FIG. 1.

Physical map of the R. sphaeroides 2.4.1 rdxBHIS operon. (A) Important restriction sites used in this study (P, PstI; A, AatII; S, SacII; SI, SacI) are shown. (B) Plasmids used in construction of the in-frame deletion mutants. Deleted regions are represented by Δ. (C) Chromosomal fragments used to complement the rdxB, rdxH, rdxI, and rdxS mutants. All DNA fragments were introduced in plasmid pBBR1MCS2 (18). Each gene of the rdxBHIS operon was expressed from the kanamycin resistance gene promoter on the plasmid.