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Complementation of rdxBHIS in-frame deletion mutants. Carotenoid composition (SE/SO ratio) of the wild-type strain, 2.4.1, and mutant strains was analyzed by HPLC as described in Materials and Methods. Strains were grown photosynthetically at a light intensity of 50 W/m2. Data are the mean values of two independent cultures. Dark blocks represent 2.4.1 and each of the rdxBHIS mutants; hatched blocks represent the complemented strain. Plasmid pBBR1MCS2 (18) was used to complement 2.4.1.
