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. 2000 Jun;182(12):3475–3481. doi: 10.1128/jb.182.12.3475-3481.2000

TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid Relevant characteristic(s) Reference or source
E. coli
 DH5αphe DH5αphe::Tn10dCm 6
 S17-1 C600::RP4-2(Tc::Mu)(Km::Tn7) thi pro hsdR recA Tra+ 35
R. sphaeroides
 2.4.1 Wild type W. Sistrom
 RDXBΔ 2.4.1 derivative, in-frame deletion in rdxB 31
 CCONΔ 2.4.1 derivative, in-frame deletion in ccoN 31
 JR112 2.4.1 derivative, in-frame deletion in rdxH This study
 JR114 2.4.1 derivative, in-frame deletion in rdxS This study
 JR117 2.4.1 derivative, in-frame deletion in rdxI This study
Plasmid
 pBluescript SK+ AprlacPOZ Stratagene
 pRK415 Tcr Mob+lacZα IncP 15
 pBBR1MCS2 Kmr 18
 pLO1 KmrsacB RP4-oriT ColE1-oriV 20
 pUI8180 Cosmid containing rdxBHIS operon 39
 pCF200Km Spr Str Kmr IncQ puc::lacZYA 19
 pUI1663 Spr Str Kmr IncQ puf::lacZYA 8
 pJR101 pBluescript SK+ :: 4.9-kb PstI fragment containing rdxBHIS operon This study
 pJR104 pLO1::1.8-kb FspI + HincII fragment containing in-frame deleted rdxI This study
 pJR106 pLO1::1.1-kb SspI + HincII fragment containing in-frame deleted rdxH This study
 pJR107 pLO1::1.7-kb NruI fragment containing in-frame deleted rdxS This study
 pJR115 pBBR1MCS2::0.6-kb SacII fragment containing rdxS gene This study
 pJR116 pBBR1MCS2::1.1-kb XbaI + SacI fragment containing rdxH gene This study
 pJR120 pBBR1MCS2::2.7-kb KpnI + HindIII fragment containing rdxI gene This study
 pJR121 pBBR1MCS2::2.1-kb HindIII + SacII fragment containing rdxB gene This study