TABLE 1.
Bacterial strains and plasmids used in this study
Strain or plasmid | Relevant characteristic(s) | Reference or source |
---|---|---|
E. coli | ||
DH5αphe | DH5αphe::Tn10dCm | 6 |
S17-1 | C600::RP4-2(Tc::Mu)(Km::Tn7) thi pro hsdR recA Tra+ | 35 |
R. sphaeroides | ||
2.4.1 | Wild type | W. Sistrom |
RDXBΔ | 2.4.1 derivative, in-frame deletion in rdxB | 31 |
CCONΔ | 2.4.1 derivative, in-frame deletion in ccoN | 31 |
JR112 | 2.4.1 derivative, in-frame deletion in rdxH | This study |
JR114 | 2.4.1 derivative, in-frame deletion in rdxS | This study |
JR117 | 2.4.1 derivative, in-frame deletion in rdxI | This study |
Plasmid | ||
pBluescript SK+ | AprlacPOZ′ | Stratagene |
pRK415 | Tcr Mob+lacZα IncP | 15 |
pBBR1MCS2 | Kmr | 18 |
pLO1 | KmrsacB RP4-oriT ColE1-oriV | 20 |
pUI8180 | Cosmid containing rdxBHIS operon | 39 |
pCF200Km | Spr Str Kmr IncQ puc::lacZYA′ | 19 |
pUI1663 | Spr Str Kmr IncQ puf::lacZYA | 8 |
pJR101 | pBluescript SK+ :: 4.9-kb PstI fragment containing rdxBHIS operon | This study |
pJR104 | pLO1::1.8-kb FspI + HincII fragment containing in-frame deleted rdxI | This study |
pJR106 | pLO1::1.1-kb SspI + HincII fragment containing in-frame deleted rdxH | This study |
pJR107 | pLO1::1.7-kb NruI fragment containing in-frame deleted rdxS | This study |
pJR115 | pBBR1MCS2::0.6-kb SacII fragment containing rdxS gene | This study |
pJR116 | pBBR1MCS2::1.1-kb XbaI + SacI fragment containing rdxH gene | This study |
pJR120 | pBBR1MCS2::2.7-kb KpnI + HindIII fragment containing rdxI gene | This study |
pJR121 | pBBR1MCS2::2.1-kb HindIII + SacII fragment containing rdxB gene | This study |