FIG. 2.

Delta-24-RGD infection and replication within PD-BM-hMSCs in vitro. A: Viral E1A and hexon proteins were seen in PD-BM-hMSCs. The PD-BM-hMSCs were infected with Delta-24-RGD (50 MOIs, 1 hour), trypsinized, and replated on chamber slides, as described in Methods. Forty-eight hours after infection, slides were immunostained with anti-E1A or antihexon antibodies. Representative PD-BM-hMSCs, PD-BM-MSC-001, showed viral E1A protein and hexon protein in cells. B: Representative electron micrographs showed viral replication in PD-BM-MSC-001. PD-BM-hMSCs were infected with Delta-24-RGD at 50 MOIs and examined at 24 and 48 hours after infection, as indicated in the figure. Virions were seen in the nucleus after 24 hours and in the cytoplasm surrounding the inclusion body (arrow) after 48 hours. h = hours.