| DNA (oligo pool 1ng/ul) | 2ul | |
| 5xHF-buffer | 5 | |
| dNTPs | 0.75ul | |
| TREG_1F(10uM) | 0.75ul | |
| TREG_1R(10uM) | 0.75ul | |
| KAPA pol | 0.5ul | |
| SYBR | 1.25ul | |
| H2O | to 25ul | |
| PCR 1 Protocol: | ||
| 95C | 3min | |
| 98C | 20s | |
| 55C | 15s | |
| 72C | 15s | |
| 72C | 1min | |
| 4C | --- | |
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