| 1st PCR: | ||
|---|---|---|
| gDNA | 12.5 - 25% of pooled material (depending on the cohort) | |
| 2 x KAPA mastermix | 12.5ul | |
| TREG_NGS_1F(10uM) | 1ul | |
| TREG_NGS_1R(10uM) | 1ul | |
| SYBR | 1.25ul | |
| H2O | to 25ul | |
| PCR protocol TREGS_NGS_1: | ||
| 95C | 3min | |
| 98C | 20s | |
| 60C | 15s | |
| 72C | 20s | |
| 72C | 1min | |
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