| 2nd PCR: | ||
|---|---|---|
| 1:50 diluted DNA template from PCR 1 | 8ul | |
| 2 x KAPA mastermix | 12.5ul | |
| TREG_NGS_2F(10uM) | lul | |
| TREG_NGS_2R(10uM) | lul | |
| SYBR | 1.25ul | |
| H2O | to 25ul | |
| PCR protocol TREGS_NGS_2: | ||
| 95C | 3min | |
| 98C | 30s | |
| 52.5C | 15s | |
| 72C | 20s | |
| 72C | 1min | |
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