FIG. 6.

Transcriptional organization of the H. pylori 69A groESL operon. (A) Northern blot analysis. Cells were first cultivated at 37°C and then heat shocked by upshift to 42°C. RNA was isolated before application of heat stress (0) and at the indicated times after the upshift. RNA samples (200 ng per lane) were separated by electrophoresis in a 1.2% gel and vacuum blotted to a nylon membrane. Hybridization was performed using a groEL-specific RNA probe. (B) Schematic drawing of groESL operon and the detected bicistronic mRNA. The putative rho-independent transcriptional terminator downstream of the operon is indicated. (C) Quantitative slot blot analysis of groEL-specific mRNA before and after upshift from 37 to 42°C. The calculated mRNA heat induction profile as determined by three independent experiments using different preparations of RNA is shown.