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Immunoblotting analysis of AcrB and AcrA produced from pAB before (A) and after (B) cross-linking with DSP. Cross-linked complexes (B) were separated by SDS-PAGE (8% gel) and analyzed by Western blotting with anti-AcrB (top) and anti-AcrA (bottom) antibodies. Positions of AcrB- and AcrA-specific complexes are indicated by arrows. M, size markers.
