TABLE 1.
Strains and plasmids used
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| E. coli strains | ||
| AG100 | argE3 thi-1 rpsL xyl mtl galK supE441 Δ(gal-uvrB) λ− | 3, 11 |
| AG102MB | Kanr, same as AG100 but marR1 acrB::kan | S. Ohsuka and H. Nikaido, unpublished data |
| AG100A | Kanr, same as AG100 but ΔacrAB::kan | 11 |
| ZK796 | Tetr, same as MC4100 but tolC::Tn10 | 4, 5 |
| Plasmids | ||
| pBAD/Myc-HisA | Ampr, cloning vector for dosage-dependent expression under arabinose-inducible PBAD promoter | Invitrogen |
| pACYC184 | Cmr Tetr, cloning vector | New England Biolabs |
| pUC151A | Ampr, pUC19 derivative carrying acrA and acrB | 8 |
| pAB | Cmr, pACYC184 derivative carrying acrA and acrB under PBAD promoter | This study |
| pUCBPA | Ampr, pUC18 derivative carrying acrB under native promoter | Q. Wang et al., unpublished data |
| pEZ4 | Ampr, pET21d(+) derivative carrying ompA-acrA-his6 under T7 promoter | 16 |
| pARA | Ampr, pUC18 derivative carrying araC and polycloning site from pBAD/Myc-HisA | This study |
| pOA | Cmr, pACYC184 derivative carrying ompA-acrA-his6 under PBAD promoter | This study |