Expression and secretion property of SARS-CoV-2 proteins
(A) Western blot image of total cell lysates for validating SARS-CoV-2 protein expression in HEK293 cells. The blots were probed with anti-Strep II tag and anti-HA (for SPK protein) antibodies.
(B) Western image of SARS-CoV-2 protein pulldown using Strep-Tactin beads and anti-HA-beads (for SPK) from the culture supernatants for validating SARS-CoV-2 protein secretion from HEK293 cells. The blots were also probed with anti-Strep II tag and anti-HA (for SPK) antibodies.