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. 2023 May 18;26(6):106929. doi: 10.1016/j.isci.2023.106929

Figure 2.

Figure 2

Secreted glycosylated ORF8 specifically induces the expression of pro-inflammatory cytokines

(A) Conditioned media containing major secreted NSP proteins did not induce pro-inflammatory cytokines in PBMCs of healthy donors.

(B) Conditioned media containing secreted ORF8 specifically induced pro-inflammatory cytokines (highlighted in yellow) IL1β, IL6, IL8, and CCL2 in PBMCs from unselected healthy donors. ∗p≤0.05.

(C) Purified highly glycosylated ORF8 (ORF8-glycolhi) from HEK293 but not unglycosylated ORF8 (ORF8-glycolnull) from E. coli stimulates PBMCs to produce pro-inflammatory cytokines IL1β, IL6, IL8 but not T cell cytokine IL2.