REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-ORF8, SARS-Cov2	MyBioResource	Cat# MBS3014575
Rabbit anti-NSP9, SARS-Cov2	Abcam	Cat#ab284037
Rabbit anti-NSP10, SARS-Cov2	R&D	Cat#MAB11049-SP
Rabbit anti-human IL1beta	Abcam	Cat#Ab216995, RRID:AB_2894877
Mouse anti-human ASC (B-3)	Santa Cruz	Sc-514414, RRID:AB_2737351
Rabbit anti-human NLRP3	Cell Signaling	Cat#15101S, RRID:AB_2722591
Rabbit anti-human Cleaved Caspase-1 (asp297)	Cell Signaling	Cat#4199S, RRID:AB_1903916
Rabbit anti-human Caspase-5	Cell Signaling	Cat#46680, RRID:AB_2799306
Rabbit anti-human Gasdermin D	Cell Signaling	Cat#97558, RRID:AB_2864253
Mouse anti-NFkB p65	Cell Signaling	Cat#6956, RRID:AB_10828935
Rabbit anti-pNFkB	Cell Signaling	Cat#3033, RRID:AB_331284
Rabbit anti-NFkB2, p105/p50	Cell Signaling	Cat#12540, RRID:AB_2687614
Rabbit anti-IkB alpha	Abcam	Cat#9242, RRID:AB_331623
Mouse anti-Strep Tag II,	Millipore	Cat#71590-3, RRID:AB_10807650
Mouse anti-HA, clone 12CA5	Sigma	Cat#11583816001, RRID:AB_514505
Mouse anti-Flag M2	Sigma	Cat#3165, RRID:AB_259529
Mouse anti-human IL1beta-BV421, clone AS10	BD Pharmingen	Cat#567791, RRID:AB_2916736
Rat Anti-Human IL-6-PE, clone MQ2-6A3	BD Pharmingen	Cat# 559331, RRID:AB_397228
Mouse anti-human CCL2-APC, clone 5D3-F7	Invitrogen	Cat#17-7099-81, RRID:AB_469500
Mouse anti-human IL8-PerCP-eFluor 710, clone 8CH	Invitrogen	Cat#46-8088-42, RRID:AB_10805748
CD3-FITC, clone OKT3	Biolegend	Cat# 317306, RRID:AB_571907
CD19-FITC, clone SJ25-C1	Biolegend	Cat# 363025, RRID:AB_2564254
CD14-APC-Cy7, clone M5E2	Biolegend	Cat# 301820, RRID:AB_493695
CD16-PE-Cy7, clone 3G8	Biolegend	Cat# 302016, RRID:AB_314216
CD56-PE-Dazzle, clone HCD56	Biolegend	Cat# 318347, RRID:AB_2563563
CD16-BUV496, clone 3G8	BD	Cat# 564653, RRID:AB_2744294
CD11c-PE-Cy7, clone B-ly6	BD	Cat# 561356, RRID:AB_10611859
CD5-BV605, clone L17F12	BD	Cat# 742550, RRID:AB_2740860
HLA-DR-BV785, clone GF6-6	BD	Cat# 564041, RRID:AB_2738559
Ghost Dye Violet 510	Tonbo	Cat# 13-0870-T100
Bacterial and virus strains
Stbl3	ThermoFisher	Cat# C7373-03
Biological samples
Plasma samples, patients with Covid-19	Mayo Clinic	N/A
PBMCs, healthy donors	Mayo Clinic	N/A
Chemicals, peptides, and recombinant proteins
ORF8, SARS-CoV2, purified from HEK293	This paper	N/A
ORF8, SARS-CoV2, purified from E. coli	This paper	N/A
Lipopolysaccharides (LPS)	Sigma	Cat# L2630
Nigericin	Sigma	Cat# N7143
MCC950	Sigma	Cat# S7809
Tranilast (SB 252218)	Selleckchem	Cat# S1439
OLT1177 (Dapansutrile)	Tocris	Cat# 6902
Deposited data
Single-cell RNA-seq	This paper	SRA: PRJNA971080
Experimental models: Cell lines
HEK293	ATCC	RRID:CVCL_0045
HEK293-F	ATCC	RRID:CVCL_6642
THP1	ATCC	RRID:CVCL_0006
THP-1, NLRP3 Knocked down	Invivogen	Cat code: Thp-dnlp
Oligonucleotides
Forward primer for IL1β qPCR: CCACAGACCTTCCAGGAGAATG	This paper	N/A
Reverse primer for IL1β qPCR: GTGCAGTTCAGTGATCGTACAGG	This paper	N/A
Forward primer for IL6 qPCR: AGACAGCCACTCACCTCTTCAG	This paper	N/A
Reverse primer for IL6 qPCR: TTCTGCCAGTGCCTCTTTGCTG	This paper	N/A
Forward primer for IL8 qPCR: GAGAGTGATTGAGAGTGGACCAC	This paper	N/A
Reverse primer for IL8 qPCR: CACAACCCTCTGCACCCAGTTT	This paper	N/A
Forward primer for CCL2 qPCR: AGAATCACCAGCAGCAAGTGTCC	This paper	N/A
Reverse primer for CCL2 qPCR: TCCTGAACCCACTTCTGCTTGG	This paper	N/A
Forward primer for beta-Actin qPCR: CACCATTGGCAATGAGCGGTTC	This paper	N/A
Reverse primer for beta-Actin qPCR: AGGTCTTTGCGGATGTCCACGT	This paper	N/A
Forward primer for GAPDH qPCR: GTCTCCTCTGACTTCAACAGCG	This paper	N/A
Reverse primer for GAPDH qPCR: ACCACCCTGTTGCTGTAGCCAA	This paper	N/A
Recombinant DNA
pLVX-EF1alpha-SARS-CoV-2-NSP1-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141367
pLVX-EF1alpha-SARS-CoV-2-NSP2-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141368
pLVX-EF1alpha-SARS-CoV-2-NSP4-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141369
pLVX-EF1alpha-SARS-CoV-2-NSP5-C145A-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141371
pLVX-EF1alpha-SARS-CoV-2-NSP7-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141373
pLVX-EF1alpha-SARS-CoV-2-NSP8-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141374
pLVX-EF1alpha-SARS-CoV-2-NSP9-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141375
pLVX-EF1alpha-SARS-CoV-2-NSP10-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141376
pLVX-EF1alpha-SARS-CoV-2-NSP12-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141378
pLVX-EF1alpha-SARS-CoV-2-NSP13-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141379
pLXV-EF1alpha-2xStrep-SARS-CoV-2-NSP14-IRES-Puro	Addgene	RRID:Addgene_141380
pLVX-EF1alpha-SARS-CoV-2-NSP15-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141381
pBOB-CAG-SARS-CoV2-SPK-HA	Addgene	RRID:Addgene_141347
pLVX-EF1alpha-SARS-CoV-2-ORF3a-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141383
pLXV-EF1alpha-2xStrep-SARS-CoV-2-ORF3b-IRES-Puro	Addgene	RRID:Addgene_141384
pLVX-EF1alpha-SARS-CoV-2-ENV-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141385
pLVX-EF1alpha-SARS-CoV-2-MEM-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141386
pLVX-EF1alpha-SARS-CoV-2-ORF6-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141387
pLVX-EF1alpha-SARS-CoV-2-ORF7a-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141388
pLXV-EF1alpha-2xStrep-SARS-CoV-2-ORF7b-IRES-Puro	Addgene	RRID:Addgene_141389
pLVX-EF1alpha-SARS-CoV-2-ORF8-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141390
pLVX-EF1alpha-SARS-CoV-2-NUC-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141391
pLVX-EF1alpha-SARS-CoV-2-ORF10-2xStrep-IRES-Puro	Addgene	RRID:Addgene_141394
pcDNA3-N-Flag-NLRP3	Addgene	RRID:Addgene_75127
pcDNA3-N-Flag-NLRP3 1-90	Addgene	RRID:Addgene_75137
pcDNA3-N-Flag-NLRP3 91-710	Addgene	RRID:Addgene_75140
pcDNA3-N-Flag-NLRP3 711-1034	Addgene	RRID:Addgene_75141
Software and algorithms
Outcyte 1.0		http://www.outcyte.com/analyse/
SignaIP 5.0		https://services.healthtech.dtu.dk/services/SignalP-5.0/
NetNGlyc 1.0		https://services.healthtech.dtu.dk/services/NetNGlyc-1.0/
NetOGlyc 4.0		https://services.healthtech.dtu.dk/services/NetOGlyc-4.0/
TMHMM 2.0		https://services.healthtech.dtu.dk/services/TMHMM-2.0/
Flowjo version 10.8.1		https://www.flowjo.com/
GraphPad Prism version 8.0		https://www.graphpad.com/
JMP version 14.0		https://www.jmp.com/