Figure 6.

OGHDL and ASNS expression profiling following repeated DNA damage versus a single dose of radiotherapy. 22Rv1 cells were treated daily with 1 μg/ml of phleomycin for 6 weeks (6WP) or left untreated (WT, AM). RNA was extracted and sequenced on the Illumina NovaSeq 6000 platform by the AGRF to generate 100 bp single end reads. RNA-seq reads for A) OGDHL and B) ASNS following repeated DNA damage. Gene expression analysis of C) OGDHL and D) ASNS via RT-qPCR 24 hours following a single dose of 2 Gy RT. The mean and SEM from three biological replicates are shown, p-value determined by students t-test. E) CBioPortal overall survival data for prostate cancer patients with altered ASNS.