Figure 4.

MiR-516b-5p inhibitor restored circ-OXCT1 depletion-mediated effects in NSCLC cells. (a) In-miR-NC or in-miR-516b-5p was transfected into A549 and H1650 cells, and the transfection efficiency was confirmed by qRT-PCR. (b-l) A549 and H1650 cells were transfected with sh-NC or sh-circ-OXCT1 and in-miR-NC or in-miR-516b-5p. (b) The expression of miR-516b-5p was tested by qRT-PCR. (c-g) CCK-8 assay (c-d), colony formation assay (e-f) and EdU assay (g) were performed to investigate the proliferation ability of NSCLC cells. (h) The cell apoptosis rate of A549 and H1650 cells was detected by flow cytometry. (i-j) The number of migrated and invaded cells was measured by transwell assay. (k-l) The protein levels of PCNA, Bax and E-cadherin were detected by western blotting. (m-n) The relative glutamine uptake and α-KG production were determined using commercial kits. n = 3 independent biological replicates. Data were showed as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 by one-ANOVA followed by Tukey’s post hoc test.