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. 2023 Mar 2;22(10):1182–1195. doi: 10.1080/15384101.2022.2071565

Figure 6.

Figure 6.

MiR-516b-5p mimic-mediated effects were reverted by SLC1A5 overexpression in NSCLC cells. (a) A549 and H1650 cells was transfected with pcDNA or SLC1A5, and the transfection efficiency was confirmed by western blotting. (b-n) MiR-NC or miR-516b-5p and pcDNA or SLC1A5 were transfected into A549 and H1650 cells. (b-c) The protein expression of SLC1A5 was detected by western blotting. (d-g) CCK-8 assay (d-e), colony formation assay (f) and EdU assay (g) were performed to investigate the proliferation ability of NSCLC cells. (h) The cell apoptosis rate of A549 and H1650 cells was detected by flow cytometry. (i-j) The number of migrated and invaded cells was measured by transwell assay. (k-l) The protein levels of PCNA, Bax and E-cadherin were detected by western blotting. (m-n) The relative glutamine uptake and α-KG production were determined using commercial kits. n = 3 independent biological replicates. Data were showed as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 by one-ANOVA followed by Tukey’s post hoc test.