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. 2023 May 8;120(20):e2217451120. doi: 10.1073/pnas.2217451120

Fig. 1.

Fig. 1.

ATG5–BST2 complex forms independently of Vpu and ahead of LC3C Recruitment. (A) Overview of the interaction between BST2, Vpu, LC3C and ATG5. (B) Immunoprecipitation of endogenous BST2 in HeLa cells extracts co-transfected with plasmids expressing Flag-ATG5, Flag-LC3C, and Flag-Beclin 1. Immunoprecipitated proteins were detected by western blotting using rabbit anti-BST2 and anti-Flag HRP antibodies. (C) Immunoprecipitation of endogenous BST2 in HeLa cells extracts co-transfected with plasmids expressing Flag-ATG5 and GFP or Vpu-GFP. Immunoprecipitated proteins were detected by western blotting using rabbit anti-BST2 and anti-Flag HRP antibodies. All western blots presented are representative of at least three independent experiments. Immunoprecipitation of endogenous BST2 were done with mouse anti-BST2 antibodies in all experiments.