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. 2023 May 8;120(20):e2218229120. doi: 10.1073/pnas.2218229120

Fig. 5.

Fig. 5.

TRAF4 promotes upregulation of selective AR-targeted CRPC-associated genes. (A) TRAF4 overexpression does not activate AR activity on classical AR targets under androgen-deprived condition. T1, low TRAF4-expressing LNCaP cells. T2, high TRAF4-expressing LNCaP cells. (B) TRAF4 overexpression up-regulates UBE2C gene expression. (C) AR depletion abolishes TRAF4-up-regulated UBE2C expression. (D) TRAF4 depletion in CRPC cells decreases UBE2C expression. LNCaP-C4-2 cells were treated with control siRNA or siTRAF4. The levels of TRAF4 and UBE2C mRNA were measured through qRT-PCR. (E) Depletion of TRAF4 or UBE2C inhibits CRPC Lymph Node Carcinoma of the Prostate-androgen ablation (LNCaP-Abl) cell growth. LNCaP-Abl cell growth was measured by counting cell numbers at 0, 7, and 10 d after siRNA transfection. *represents P < 0.05.