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. 2023 May 8;120(20):e2303487120. doi: 10.1073/pnas.2303487120

Fig. 2.

Fig. 2.

CteG interacts with CETN2 under infection and transfection conditions. (A) Co-IP of FLAG-tagged effectors from CETN2-HA transfected HeLa cells infected with C.t. expressing the FLAG-tagged effectors. (B) Co-IP of HA-tagged CETN2 from HeLa cells cotransfected with GFP-tagged effectors and CETN2-HA. (C) Immunofluorescence images of HeLa cells cotransfected with CETN2-dsRed (red) and empty vector-, CteG-, or TmeA- GFP (green). Nucleus is stained with DAPI (blue) (Scale bar, 10 microns). Pearson Correlation Coefficients (R Value) were calculated using ImageJ Coloc2 function. Significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Error bars are SD, ****P < 0.0001. (D) Co-IP of purified CETN2-GST and MBP-tagged effectors proteins. Coomassie showing expression of MBP tagged proteins. * indicate correct molecular weight. Data are representative of two to three replicates.