Fig. 4.

CETN2 is necessary for CteG-mediated centrosome amplification in chlamydia-infected cells. (A) Representative images of HeLa cells depleted (CETN2 KD, Bottom) or not (Control KD, Top) of CETN2 and infected with WT L2 or cteG::aadA for 36 h. Cells were stained with C.t. HSP-60 (red), pericentrin (green), and DAPI (blue). White arrows indicate centrosomes (Scale bar, 10 microns). (B) Quantification of cells with supernumerary centrosomes (>2) at 36 h postinfection in CETN2 KD (pink) and control KD (blue) cells. Error bars are SD, *P < 0.05, **P < 0.01. Significance was determined using two-way ANOVA followed Tukey’s multiple comparisons test. Data are representative of two replicates.