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. 2023 May 8;120(20):e2303487120. doi: 10.1073/pnas.2303487120

Fig. 5.

Fig. 5.

Intact C-terminus of CETN2 is needed for CteG–CETN2 interaction. (A) Schematic of CETN2 truncations made with full-length CETN2 at the Top. Pink boxes represent EF-hand domains. Gray circles indicate intact calcium-binding domains. (B and C) Co-IP of FLAG-tagged CteG from HeLa cells transfected with N- or C-terminal truncations of CETN2 and infected with C.t. expressing the FLAG-tagged CteG. (D) Schematic of CETN2 mutations made to phosphorylation site S170 and the calcium-binding domain of EF hand 4. Top figure shows intact calcium-binding domains and S170 phosphorylation site (green circle). (E) Co-IP of HA-tagged CETN2 mutants from HeLa cells cotransfected with GFP-tagged CteG and CETN2 mutants. Data are representative of two to three replicates.