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. 2023 May 8;120(20):e2301137120. doi: 10.1073/pnas.2301137120

Fig. 1.

Fig. 1.

A tessellated lymphoid network supports coordinated whole-body T cell trafficking in juvenile and adult zebrafish. (A) The organism-scale organization of T cells in a 10 wpf tg(lck:GFP) zebrafish, with the inset showing the hexagonal "zigzag" pattern. (B) The emergence of the T cell network is shown with representative images of the caudal peduncle of tg(lck:GFP) zebrafish at 3, 5, 7, 10, and 15 wpf. (C) Imaging of the caudal peduncle shows the organization of T cells (lck:GFP), macrophages (mpeg1:mCherryCAAX), and neutrophils (lyzc:BFP) in triple transgenic zebrafish. (D and E) Imaging of the caudal peduncle at low (d) or high (e) magnification shows the presence of dual labeled cells in tg(lck:GFP;cd4-1:mCherry) fish. MΦ = macrophage, L.E. = leading edge, Ur. = uropod. (F) Timelapse imaging of T cells migrating through the TLN over 20 min, with the cell tracks and directions of eight representative GFP+ cells shown in magenta. (Scale bar = 100 microns.) (G) Plots show the cell tracks of GFP+ cells migrating in the TLN of tg(lck:GFP;cd4-1:mCherry) in the indicated regions of five individual fish pooled from three independent experiments. Each dot = average per fish, asterisks = average total movement. Scale is in microns. (H) A summary of the movement of T cells in the TLN.