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Dissociation constant (Kd) determination of FMDV1 aptamer. The saturation curve was obtained by plotting the aptamer concentration (nM) versus the total ssDNA (ng) bound to 3ABC protein. Protein-bound aptamer was quantified by qPCR. The Kd was calculated by non-linear regression analysis (One-Site Specific Binding with Hill slope software GraphPad Prism 5), resulting in Kd = 22.69 ± 1.79 nM.
