Fig. 3.
BHK-21 cells express type-1 IFN (α/β) during RO8191 treatment. A) Percentage of CHIKVWT infection by measuring mRNA-CHIKVWT in supernatant of BHK-21 cells. B) CHIKVWT RNA titers by measuring mRNA-CHIKVWT in supernatant of BHK-21 cells. C) Percentage of CHIKVWT infection by measuring mRNA-CHIKVWT per mL in cell lysates of BHK-21 cells. D) CHIKVWT RNA titers by measuring mRNA-CHIKVWT per mL detected in cell lysates of BHK-21 cells. The CHIKV infection is presented in black bars while CHIKV in the presence of RO8191 treatment is demonstrated in gray bars. E) mRNA means of IFN-α expressed by BHK-21 during infection and/or treatment with RO8191. F) mRNA mean of IFN-β expressed by BHK-21 during infection and/or treatment with RO8191. BHK-21 cells were infected with CHIKVWT (MOI 0.1 PFU/well) in the presence or absence of RO8191 at 5 µM for 16 h. The supernatant and cell lysates were collected and processed to RNA extraction. cDNA was produced and mRNA levels was quantified by RT-qPCR. Mean values of three independent experiment each measured in triplicate, are represented. (****) P <0.0001. Images were generated using GraphPad Prism 8 and GIMP 2.1v.