Fig. 3.

Oncolytic virus O-HSV1 inhibits proliferation and promotes apoptosis of SW620 cells. A Cell viability was measured by CCK8 assay after infection with different viruses at an MOI of 1. Uninfected cells were considered as 100% viable (n = 3). B Quantitative RT‒PCR analysis of Bax, Bcl2, Casp3 and Casp9 mRNA in SW620 cells infected with D-34.5 and O-HSV1 at an MOI of 1 for 48 h. C SW620 cell apoptosis induced by different virus was analyzed by Annexin V-FITC and PI staining. (n=3). ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (two-tailed Student's t test).