Fig. 1.

IRF3 deficiency impairs innate antiviral immune response to TMEV. 1 × 10⁶ RAW Lucia or IRF3KO-RAW Lucia cells were incubated overnight in 6-well plates and then challenged with 5 × 10⁶ TMEV. After 18 and 24 h, TMEV RNA (A), IL-33 (B) and IFN-beta (C) mRNA by qRT-PCR, secreted IL-6 (E), IL-10 (F) and IL-33 (G, H) by ELISA. For the reported experiment n=3 for each condition, which was repeated n=3. Data are means ± standard error and were analyzed by Student's t-test; * indicates significantly different than control, p≤ 0.05.