Fig. 3.
Blockade of IL-33RA ST2 alters M1/M2 phenotypic markers during TMEV infection. 1 × 106 RAW Lucia cells were incubated overnight in 6-well plates and then challenged with 5 × 106 TMEV with and without 10 μg/ml monoclonal antibody to IL-33R ST2. After 24 h, mRNA expression of TMEV, IL-6 (M1 marker) or CD206 and ARG1 (M2 markers) was evaluated by qRT-PCR. For the reported experiment n=3 for each condition, which was repeated n=3. Data are means ± standard error and were analyzed by Student's t-test; * indicates significantly different than control, p≤ 0.05.