Fig. 4.
IL-33 treatment increases TMEV infection of RAW cells. 1 × 106 RAW Lucia or IRF3KO RAW Lucia cells were incubated overnight in 6-well plates, pretreated with 20 ng/ml IL-33 for 24 h, and then challenged with 5 × 106 TMEV. After 24 h, cells were assessed for TMEV RNA (A), IL-33 (B), IFN-beta (C), and IL-10 (D) mRNA using qRT-PCR. For the reported data n=3 for each condition of an experiment, which was repeated n=3. Data are means ± standard error analyzed by Student's t-test; *; ** indicates significantly different from controls, p≤ 0.05.