Fig. 7.

Induced IL-33 gene expression in IL-33 pretreated RAW cells is IRF3 dependent. (A) 1 × 10⁶ RAW Lucia or IRF3KO RAW Lucia cells were incubated overnight in 6-well plates, pretreated with 20 ng/ml IL-33 for 24 h, and then stimulated with 10 μg/ml poly I:C (IC) with or without 20 ng/ml IFN-gamma (IFN-γ). (B) 2 × 10⁵ IRF3KO RAW Lucia cells were incubated overnight in 24-well plates and then cells were transfected with 0.5 μg per well pEGFP Empty vector or pIRF3 expression vector. After 24 h of transfection, cells were stimulated with 10 ug/ml poly I:C (IC) with or without 20 ng/ml IFN-gamma (IFN-g). After 24 h of stimulation, qRT-PCR assessed IL-33 expression (A, B). For A, n=3 for each condition of an experiment, which was repeated n=3 and for B, n=4. Data are means ± standard error and were analyzed by Student t test; * indicates significantly different from controls (nil), p≤ 0.05.