FIGURE 2.

Repolarization power of wild‐type (WT) hERG as a function of temperature in HEK293 cells transiently expressing hERG. A model action potential was applied (action potential [AP]‐clamp) using a conventional patch clamp. (A) Typical current recordings on a single cell during AP‐clamp at various temperatures. Dashed line: time course of the model AP³¹ (voltage scale: right Y axis). (B) Current recordings during AP‐clamp at 22°C (same cell as A) for AP of various durations (inset). (C) Corresponding repolarization power at various temperatures versus time factor. Horizontal dashed line: reference repolarization power at 32°C: 10.9 pA/pF.s for this cell. (D) As in B, at 22°C, after time and current density corrections using various factors from 1.5 to 3 on the respective recordings. For the recordings obtained during the AP of x 2 duration, the time has been divided by 2 and the current density multiplied by 2. Again, note, as in Figure 1D, the overlap of the current at 22°C, corrected by 2 (blue), with the reference current obtained during the standard AP at 32°C (orange). (E) Tukey plot of the repolarization powers recorded at 32 and 22°C during the standard AP or AP of doubled duration (n = 9) versus 32°C: ns: non‐significant; **: p < 0.01.