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Nonenzymic, FADH2-dependent reduction of cob(III)alamin to cob(II)alamin. UV visible spectra, labeled as follows: 1, cob(III)alamin alone; 2, FADH2; 3, spectrum of the solution containing FADH2 obtained immediately after the addition of cob(III)alamin to it; 4, same as spectrum 3, but the spectrum was obtained 2 min after the addition of cob(III)alamin. The inset shows overlaid spectra of FAD (labeled A) and authentic cob(II)alamin (labeled B).
